University of Arkansas for Medical Sciences

3 ARTICLES PUBLISHED IN JoVE

Biology

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Lauren P. Blair¹, Nathan L. Avaritt¹, Alan J. Tackett¹

¹Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences

We present a method for using MALDI mass spectrometry and reductive methylation chemistry to quantify changes in lysine methylation.

Immunology and Infection

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Xuelian Wang¹, William W. Greenfield², Hannah N. Coleman³, Lindsey E. James³, Mayumi Nakagawa³

¹Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University , ²Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences , ³Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences

Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.

Biology

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Grażyna Nowak¹, Diana Bakajsova¹

¹Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences

The effects of activation of protein kinase C (PKC) isozymes on mitochondrial functions associated with respiration and oxidative phosphorylation and on cell viability are described. The approach adapts adenoviral technique to selectively overexpress PKC isozymes in primary cell culture and a variety of assays to determine mitochondrial functions and energy status of the cell.