Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

12.3K Views

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13:41 min

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March 8th, 2012

DOI :

10.3791/3657-v

March 8th, 2012

•

12,254 Views

Xuelian Wang¹, William W. Greenfield², Hannah N. Coleman³, Lindsey E. James³, Mayumi Nakagawa³

¹Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University , ²Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences , ³Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences

Chapters

Summary

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Interferon gamma

Enzyme linked Immunospot Assay

T cell Epitopes

Human Papillomavirus HPV

Protocol

Isolating And Characterizing Rare T Cells

HPV Proteins

Peptide specific T Cells

Loop Electrical Excision Procedure

High grade Squamous Intraepithelial Lesion

Human Papillomavirus Type 16 HPV 16

E6 And E7 Proteins

IFN g Enzyme linked Immunospot ELISPOT Assay

Synthetic Peptides

Epitope Specific T Cells

CD8 T cell Isolation Kit

Miltenyi Biotec

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