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Tulane University School of Medicine

8 ARTICLES PUBLISHED IN JoVE

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Neuroscience

The Mouse Forced Swim Test
Adem Can 1, David T. Dao 2, Michal Arad 1, Chantelle E. Terrillion 3, Sean C. Piantadosi 1, Todd D. Gould 1,3,4
1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 4The Program in Neuroscience, University of Maryland

The forced swim test is validated as an experimental approach to assess potential antidepressant efficacy in rodents. Experimental animals are placed in a tank of water and escape-related mobility behavior is quantified. The common procedures for the mouse version of this test are described.

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Neuroscience

The Tail Suspension Test
Adem Can *1, David T. Dao *1,2, Chantelle E. Terrillion 3, Sean C. Piantadosi 1, Shambhu Bhat 1, Todd D. Gould 1,3,4
1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3The Program in Neuroscience, University of Maryland , 4Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine

The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.

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Biology

Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis
Ming Yang 1, Peter C. Stapor 1, Shayn M. Peirce 2, Aline M. Betancourt 3, Walter L. Murfee 1
1Department of Biomedical Engineering, Tulane University, 2Department of Biomedical Engineering, University of Virginia , 3Center for Stem Cell Research and Regenerative Medicine, Tulane University

This article describes a simple model for stimulating angiogenesis in the rat mesentery. The model produces dramatic increases in capillary sprouting, vascular area and vascular density over a relatively short time course in a tissue that allows en face visualization of entire microvascular networks down to the single cell level.

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Bioengineering

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
Ryan W. Bonvillain 1,2, Michelle E. Scarritt 1, Nicholas C. Pashos 1, Jacques P. Mayeux 1, Christopher L. Meshberger 1, Aline M. Betancourt 1,3, Deborah E. Sullivan 1,3, Bruce A. Bunnell 1,2,4
1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine

Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.

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Immunology and Infection

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
Junko Yano 1, Elizabeth A. Lilly 2, Mairi C. Noverr 2, Paul L. Fidel 1
1Center for Oral and Craniofacial Biology, Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology, Tulane University School of Medicine

Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.

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Cancer Research

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
Loren M. Brown 1, Katherine L. Hebert 2, Rakesh R. Gurrala 3, C. Ethan Byrne 4, Matthew Burow 5, Elizabeth C. Martin 4, Frank H. Lau 1
1Department of Surgery, Louisiana State University Health Sciences Center, 2Department of Bioinnovation, Tulane University, 3Tulane University School of Medicine, 4Department of Biological and Agricultural Engineering, Louisiana State University, 5Department of Medicine, Tulane University School of Medicine

This protocol describes the construction of an in vitro microphysiological system for studying breast cancer using primary human breast tissue with off the shelf materials.

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Cancer Research

Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
Shangyu Gong *1, Yichi Zhang *1, Hongcun Bao 1, Xianfeng Wang 1, Chih-Hsuan Chang 1, Yi-Chun Huang 1, Wu-Min Deng 1
1Department of Biochemistry and Molecular Biology, Tulane University School of Medicine

This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.

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Bioengineering

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
Meagan J. Makarcyzk 1,2, Zhong Alan Li 1,3, Ilhan Yu 1, Haruyo Yagi 1, Xiurui Zhang 1, Lauren Yocum 1, Eileen Li 1, Madalyn R. Fritch 1, Qi Gao 4, Bruce A. Bunnell 5, Stuart B. Goodman 4,6, Rocky S. Tuan 1,8, Peter G. Alexander 1,7, Hang Lin 1,2,7
1Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 2Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology, University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery, Stanford University, 5Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, 6Department of Bioengineering, Stanford University, 7McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong

We provide detailed methods for generating four types of tissues from human mesenchymal stem cells, which are used to recapitulate the cartilage, bone, fat pad, and synovium in the human knee joint. These four tissues are integrated into a customized bioreactor and connected through microfluidics, thus generating a knee joint-on-a-chip.

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