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Stanford University School of Medicine

9 ARTICLES PUBLISHED IN JoVE

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Biology

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds
Brendan Carvalho 1, David J. Clark 1, David Yeomans 1, Martin S. Angst 1
1Department of Anesthesiology, Stanford University School of Medicine

A technique to collect and measure surgical wound biochemical mediators at specific time points.

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Biology

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators
Martin S Angst 1, Martha Tingle 1, Martin Schmelz 2,3, Brendan Carvalho 1, David C Yeomans 1
1Department of Anesthesia, Stanford University School of Medicine, 2Department of Anaesthesiology, University of Mannheim, 3Department of Anaesthesiology, University of Heidelberg

A technique is presented for the in-vivo collection of interstitial fluid samples from pertinent tissue sites (here, experimentally inflamed skin) for the measurement of biochemicals mediating pain and inflammation.

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Biology

Determining heat and mechanical pain threshold in inflamed skin of human subjects
Martin S Angst 1, Martha Tingle 1, Nicholas G Phillips 1, Brendan Carvalho 1
1Department of Anesthesia, Stanford University School of Medicine

Algorithms assessing heat and mechanical pain thresholds in experimentally inflamed skin of human study subjects are shown. The two pain testing paradigms independently examine nociceptive processing by the two major peripheral nerve fiber populations transmitting pain, i.e., non-myelinated C fibers and small myelinated A-delta fibers.

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Medicine

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)
Ruth Landau 1, John C. Kraft 1, Lisa Y. Flint 1, Brendan Carvalho 1, Philippe Richebé 1, Monica Cardoso 1, Patricia Lavand'homme 1, Michal Granot 1, David Yarnitsky 1, Alex Cahana 1
1Department of Anesthesiology and Pain Medicine, University of Washington School of Medicine

Diffuse noxious inhibitory control, temporal summation and wound hyperalgesia testing are demonstrated in the obstetric patient. These tests evaluate inhibitory and excitatory mechanisms of pain processing and are here utilized to evaluate endogenous analgesia at different time-points during pregnancy and the peripartum period to help reveal individual s risk for persistent pain.

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Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis
Xiaoqin Hua 1,2, Tobias Deuse 1,2, Evangelos D. Michelakis 3, Alois Haromy 3, Phil S. Tsao 4, Lars Maegdefessel 4, Reinhold G. Erben 5, Claudia Bergow 5, Boris B. Behnisch 6, Hermann Reichenspurner 1,2, Robert C. Robbins 7, Sonja Schrepfer 1,2,7
1University Heart Center Hamburg, TSI-Lab, Germany, 2Cardiovascular Research Center, University of Hamburg, 3Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, 4Department of Medicine, Stanford University School of Medicine , 5Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, 6Translumina GmbH, Hechingen, 7Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

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Biology

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Wei-Meng Woo *1, Scott X. Atwood *1, Hanson H. Zhen 1, Anthony E. Oro 1
1Program in Epithelial Biology, Stanford University School of Medicine

Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

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Medicine

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique
Timothy C. Chang 1,2, Jen-Jane Liu 1,2, Joseph C. Liao 1,2
1Department of Urology, Stanford University School of Medicine , 2Veterans Affairs Palo Alto Health Care System

Probe-based confocal laser endomicroscopy enables real-time microscopy of the human urinary tract during cystoscopy, providing dynamic, intravital imaging of pathological states such as bladder cancer with cellular resolution. Endomicroscopy may augment the diagnostic accuracy of standard white light endoscopy and provide intraoperative image guidance to improve surgical resection.

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Medicine

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds
Brendan Carvalho 1, David J Clark 1, David Yeomans 1, Martin S Angst 1
1Department of Anesthesia, Stanford University School of Medicine

This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and neuropetides.

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Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models
Mandy Stubbendorff *1,2, Xiaoqin Hua *1,2, Tobias Deuse 1,2,3, Ziad Ali 4,5, Hermann Reichenspurner 2,3, Lars Maegdefessel 6, Robert C. Robbins 7, Sonja Schrepfer 1,2,3,4
1Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center, University Hospital Hamburg, 2Cardiovascular Research Center (CVRC) and DZHK University Hamburg, 3Department of Cardiovascular Surgery, University Heart Center Hamburg, 4Center for Interventional Vascular Therapy, Division of Cardiology, Columbia University, 5Cardiovascular Research Foundation, New York, 6Karolinska Institute, Stockholm, 7Department of Cardiothoracic Surgery, Stanford University School of Medicine, Falk Cardiovascular Research Center

This video shows two models of intimal plaque development in murine arteries and emphasizes the differences in myointimal hyperplasia and atherosclerosis.

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