National Institute of Immunology

4 ARTICLES PUBLISHED IN JoVE

Medicine

Protocol for Long Duration Whole Body Hyperthermia in Mice

Vikas Duhan^*1, Neha Joshi^*1, P. Nagarajan², Pramod Upadhyay¹

¹Product Development Cell, National Institute of Immunology, ²Small Animal Facility, National Institute of Immunology

This paper describes a protocol for whole body hyperthermia in mice that can stimulate fever like conditions up to 12-24 hr.

Medicine

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Barun Das¹, Jashdeep Bhattacharjee¹, Preeti¹, Alaknanda Mishra¹, Kshama Jain¹, Srikanth Iyer¹, Ashwani Kesarwani¹, Parul Sahu¹, Prakriti Sinha¹, Perumal Nagarajan¹, Pramod Upadhyay¹

¹National Institute of Immunology

We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdc^scid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.

Medicine

Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen

Preeti Sahay¹, Kshama Jain¹, Prakriti Sinha¹, Barun Das¹, Alaknanda Mishra¹, Ashwani Kesarwani¹, Parul Sahu¹, K. Varsha Mohan¹, M.J. Mahesh Kumar², Perumal Nagarajan¹, Pramod Upadhyay¹

¹National Institute of Immunology, ²Center for Cellular and Molecular Biology

The acute liver failure animal model developed in the current study presents a feasible alternative for the study of potential therapies. The current model employs the combined effect of physical and drug-induced hepatic injury and provides a suitable time window to study the potential of novel therapies.

JoVE Core

Microdissection of the Rodent Eye

K. Varsha Mohan¹, Prakriti Sinha¹, Bharati Swami¹, Abaranjitha Muniyasamy¹, Perumal Nagarajan¹, Pramod Upadhyay¹

¹National Institute of Immunology

This paper presents a protocol for ocular micro-dissection in rodents. The process involves the enucleation of the eyeball along with the nictitating membrane (i.e., the third eyelid). This is then followed by the separation of the posterior and anterior eye cups.