Enucleation of the Mouse Eye Along with the Third Eyelid
4:03
Clearing the Extraneous Tissue
5:17
Dissection of the Enucleated Eye to Obtain Anterior and Posterior Eyecups
7:28
Isolation of Neural Retina and RPE Layers
8:16
Segmentation of Retinal and RPE Layers for Whole Mounts
9:38
Results
10:12
Conclusion
Transcript
Ocular research in the earlier times mostly relied on the ocular tissue from deceased individuals. However, over the years, many ophthalmic rodent models have been developed and the need for human ocular tissue has diminished. Due to the small siz
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This paper presents a protocol for ocular micro-dissection in rodents. The process involves the enucleation of the eyeball along with the nictitating membrane (i.e., the third eyelid). This is then followed by the separation of the posterior and anterior eye cups.