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Technion-Israel Institute of Technology

10 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Visualization of Proprioceptors in Drosophila Larvae and Pupae
Naomi Halachmi 1, Atalya Nachman 1, Adi Salzberg 1
1Department of Genetics and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

A method to immunostain and visualize chordotonal organs in larvae and pupae of Drosophila melanogaster is described.

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Biology

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
Boris Shklyar 1, Jeny Shklover 1, Estee Kurant 1
1Department of Anatomy and Cell Biology and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

Here we describe an effective method for studying dynamics of apoptotic cell clearance in vivo. This method employs live Drosophila embryos as a powerful model for monitoring phagocytosis of apoptotic cells using specific labeling of apoptotic cells and phagocytes.

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Bioengineering

Engineered Vascularized Muscle Flap
Dana Egozi *1, Yulia Shandalov *2, Alina Freiman 2,3, Dekel Rosenfeld 2, David Ben-Shimol 4, Shulamit Levenberg 2
1Department of Plastic Surgery, Kaplan Medical Center, 2Biomedical Engineering, Technion Israel Institute of Technology, 3Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, 4Medicine Department, Technion Israel Institute of Technology

To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.

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Biology

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Yulia Fridman 1, Neta Holland 1, Rivka Elbaum 2, Sigal Savaldi-Goldstein 1
1Faculty of Biology, Technion-Israel Institute of Technology, 2Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem

Crystalline cellulose is an important constituent of the plant cell wall. However, its quantification at a cellular resolution is technically challenging. Here, we report the use of polarized light technology and root cross sections to obtain information of cell wall composition at a spatiotemporal resolution.

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Genetics

An Assay for Quantifying Protein-RNA Binding in Bacteria
Noa Katz *1, Roni Cohen *1, Orna Atar 1, Sarah Goldberg 1, Roee Amit 1,2
1Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, 2Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology

In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.

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Bioengineering

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
Omer Adir *1,2, Noga Sharf-Pauker *1,2, Gal Chen *1,3, Maya Kaduri 1, Nitzan Krinsky 1,3, Janna Shainsky-Roitman 1, Jeny Shklover 1, Avi Schroeder 1
1Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering, Technion - Israel Institute of Technology, 2The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology, Technion - Israel Institute of Technology, 3The Interdisciplinary Program for Biotechnology, Technion - Israel Institute of Technology

This protocol describes the method, materials, equipment and steps for bottom-up preparation of RNA and protein producing synthetic cells. The inner aqueous compartment of the synthetic cells contained the S30 bacterial lysate encapsulated within a lipid bilayer (i.e., stable liposomes), using a water-in-oil emulsion transfer method.

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Medicine

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects
Tal Capucha *1, Dekel Shilo *1,2, Ori Blanc 1, Shahar Turgeman 1, Omri Emodi 1,2, Adi Rachmiel 1,2
1Department of Oral and Maxillofacial Surgery, Rambam Medical Care Center, 2Ruth & Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

This protocol describes the use of 3D planning and printing for reconstruction of bony defects. We use segmentation tools to create 3D models followed by 3D design software to create patient specific implants for reconstruction purposes concomitant to ablative surgery or as a second stage.

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Medicine

Treatment of Facial Deformities using 3D Planning and Printing of Patient-Specific Implants
Dekel Shilo *1,2, Tal Capucha *1, Dana Goldstein 3, Yekaterina Bereznyak 3, Omri Emodi 1,2, Adi Rachmiel 1,2
1Department of Oral and Maxillofacial Surgery, Rambam Medical Care Center, 2Ruth & Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, 3Department of Orthodontics and Craniofacial Anomalies, School of Graduate Dentistry, Rambam Health Care Campus

As technology develops and becomes more user-friendly, planning of operations and patient-specific surgical guides and fixation plates should be performed by the surgeon. We present a protocol for 3D planning of orthognathic skeletal movements and 3D planning and printing of patient-specific fixation plates and surgical guides.

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Bioengineering

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
Ariel A. Szklanny 1, Dylan B. Neale 2, Joerg Lahann 2, Shulamit Levenberg 1
1Faculty of Biomedical Engineering, Technion, 2Department of Chemical Engineering and Biointerfaces Institute, University of Michigan, Ann Arbor

Engineered tissues heavily rely on proper vascular networks to provide vital nutrients and gases and remove metabolic waste. In this work, a stepwise seeding protocol of endothelial cells and support cells creates highly organized vascular networks in a high-throughput platform for studying developing vessel behavior in a controlled 3D environment.

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Bioengineering

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
Majd Machour 1, Ariel A. Szklanny 1, Shulamit Levenberg 1
1Faculty of Biomedical Engineering, Technion-Israel Institute of Technology

Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.

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