Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

10.9K Views

•

09:37 min

•

April 27th, 2020

DOI :

10.3791/60829-v

April 27th, 2020

•

Omer Adir*¹^,², Noga Sharf-Pauker*¹^,², Gal Chen*¹^,³, Maya Kaduri¹, Nitzan Krinsky¹^,³, Janna Shainsky-Roitman¹, Jeny Shklover¹, Avi Schroeder¹

¹Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering, Technion - Israel Institute of Technology, ²The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology, Technion - Israel Institute of Technology, ³The Interdisciplinary Program for Biotechnology, Technion - Israel Institute of Technology

Summary

Explore More Videos

Centrifugation Protocol

Liposome Preparation

Advanced Drug Delivery

Chapters in this video

0:04

Introduction

0:48

Preparation of S30-T7 Lysate

4:24

Preparation of Lipids-in-Oil Solution

5:08

Preparation of Synthetic Cells

7:24

Results: Production of sfGFP and Renilla Luciferase

8:57

Conclusion

Related Videos

13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

16.9K Views

08:58

Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device

16.0K Views

10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

8.8K Views

09:16

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

10.0K Views

10:43

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

39.3K Views

08:52

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

17.3K Views

07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

5.7K Views

07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

2.6K Views

06:08

From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

4.3K Views

05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

3.3K Views