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University of Colorado Boulder

17 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
Leslie A. Morton *1,2, Jonel P. Saludes *1,2, Hang Yin 1,2
1Department of Chemistry & Biochemistry, University of Colorado Boulder, 2Biofrontiers Institute, University of Colorado Boulder

This protocol describes an extrusion method for preparing lipid vesicles of sub-micron sizes with a high degree of homogeneity. This method uses a pressure-controlled system with controlled nitrogen flow rates for liposome preparation. The lipid preparation1,2, liposome extrusion, and size characterization will be presented herein.

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Environmental Sciences

Physical Properties Of Minerals II: Polymineralic Analysis
Alan Lester 1
1University of Colorado Boulder

Physical Properties Of Minerals II: Polymineralic Analysis

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Environmental Sciences

Physical Properties Of Minerals I: Crystals and Cleavage
Alan Lester 1
1University of Colorado Boulder

Physical Properties Of Minerals I: Crystals and Cleavage

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Environmental Sciences

Igneous Intrusive Rock
Alan Lester 1
1University of Colorado Boulder

Igneous Intrusive Rock

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Environmental Sciences

Igneous Volcanic Rock
Alan Lester 1
1University of Colorado Boulder

Igneous Volcanic Rock

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Environmental Sciences

Determining Spatial Orientation of Rock Layers with the Brunton Compass
Alan Lester 1
1University of Colorado Boulder

Determining Spatial Orientation of Rock Layers with the Brunton Compass

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Environmental Sciences

Using Topographic Maps to Generate Topographic Profiles
Alan Lester 1
1University of Colorado Boulder

Using Topographic Maps to Generate Topographic Profiles

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Environmental Sciences

Making a Geologic Cross Section
Alan Lester 1
1University of Colorado Boulder

Making a Geologic Cross Section

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Neuroscience

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
Shinya Nakamura *1,2, Michael V. Baratta *1,2, Donald C. Cooper 1,2
1Institute for Behavioral Genetics, University of Colorado Boulder, 2Department of Psychology and Neuroscience, University of Colorado Boulder

The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.

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JoVE Core

Handwriting Analysis Indicates Spontaneous Dyskinesias in Neuroleptic Naïve Adolescents at High Risk for Psychosis
Derek J. Dean 1, Hans-Leo Teulings 2, Michael Caligiuri 3, Vijay A. Mittal 1
1Department of Psychology and Neuroscience, Center for Neuroscience, University of Colorado Boulder, 2NeuroScript LLC, 3Department of Psychiatry, University of California, San Diego

Handwriting analysis software significantly improves upon existing instruments measuring movement disorders. Individuals at risk for psychosis and healthy controls completed handwriting tasks to test for dyskinesia. Results suggest that youth at risk for psychosis exhibit dyskinesia and that handwriting analysis could significantly contribute to wider dissemination of early identification efforts

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JoVE Journal

Atom Transfer Radical Polymerization of Functionalized Vinyl Monomers Using Perylene as a Visible Light Photocatalyst
Jordan C. Theriot 1, Matthew D. Ryan 1, Tracy A. French 1, Ryan M. Pearson 1, Garret M. Miyake 1
1Department of Chemistry and Biochemistry, University of Colorado Boulder

A method for the atom transfer radical polymerization of functionalized vinyl monomers using perylene as a visible-light photocatalyst is described.

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Behavior

Behavioral Assessment of Hearing in 2 to 4 Year-old Children: A Two-interval, Observer-based Procedure Using Conditioned Play-based Responses
Angela Yarnell Bonino 1,2, Lori J. Leibold 3
1Department of Allied Health Sciences, The University of North Carolina at Chapel Hill, 2Department of Speech, Language, and Hearing Sciences, University of Colorado Boulder, 3Center for Hearing Research, Boys Town National Research Hospital

This paper describes a procedure for measuring hearing sensitivity in 2 to 4 year-old children. Children are trained to perform play-based responses when they hear a target signal. Thresholds are then estimated in a two-interval, two-alternative forced-choice paradigm, based on observations of the child's behaviors.

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Biology

Protocol for Analysis and Consolidation of TrackMate Outputs for Measuring Two-Dimensional Cell Motility using Nuclear Tracking
Graycen E. Wheeler 1, Amrita Purkayastha 1, Eric N. Bunker 1, David M. Bortz 2, Xuedong Liu 1
1Department of Biochemistry, University of Colorado Boulder, 2Department of Applied Mathematics, University of Colorado Boulder

This protocol for high-throughput measurement of cell motility in HaCaT keratinocytes describes methods for collecting and processing images of cell nuclei and performing particle tracking using the ImageJ plugin TrackMate.

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Bioengineering

Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
Victoria G. Muir 1, Margaret E. Prendergast 1, Jason A. Burdick 1,2,3
1Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, 2BioFrontiers Institute, University of Colorado Boulder, 3Department of Chemical and Biological Engineering, College of Engineering and Applied Science, University of Colorado Boulder

This work describes straightforward, adaptable, and low-cost methods to fabricate microgels with extrusion fragmentation, process the microgels into injectable granular hydrogels, and apply the granular hydrogels as extrusion printing inks for biomedical applications.

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Bioengineering

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
Jessica J. Senior *1, Richard J. A. Moakes *2, Megan E. Cooke 3, Samuel R. Moxon 4, Alan M. Smith 1, Liam M. Grover 2
1Department of Pharmacy, University of Huddersfield, 2School of Chemical Engineering, University of Birmingham, 3Chemical and Biological Engineering, University of Colorado Boulder, 4School of Biological Sciences, University of Manchester

Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.

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Biology

Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs
Catalina S. Bastías 1, Lea M. Savard 2, Kevin N. Eckstein 2, Kathleen Connell 3, Callan M. Luetkemeyer 2,5, Virginia L. Ferguson 1,2,4, Sarah Calve 1,2,4
1Biomedical Engineering Program, University of Colorado Boulder, 2Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, 3Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 4BioFrontiers Institute, University of Colorado Boulder, 5Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign

This article presents a detailed protocol for dissecting uterosacral ligaments and other pelvic floor tissues, including the cervix, rectum, and bladder in mice, to expand the study of female reproductive tissues.

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Bioengineering

3D Printing Bacteria to Study Motility and Growth in Complex 3D Porous Media
R. Kōnane Bay 1,2, Anna M. Hancock 2, Arabella S. Dill-Macky 2, Hao Nghi Luu 2, Sujit S. Datta 2
1Department of Chemical and Biological Engineering, University of Colorado Boulder, 2Department of Chemical and Biological Engineering, Princeton University

This protocol describes a procedure for three-dimensional (3D) printing of bacterial colonies to study their motility and growth in complex 3D porous hydrogel matrices that are more akin to their natural habitats than conventional liquid cultures or Petri dishes.

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