Queen's University

2 ARTICLES PUBLISHED IN JoVE

Biology

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

James A. Kaupp¹, Joanna F. Weber¹, Stephen D. Waldman^1,2

¹Department of Mechanical and Materials Engineering, Queen's University , ²Department of Chemical Engineering, Queen's University

The biosynthesis of cartilaginous extracellular matrix by chondrocytes can be affected by application of mechanical stimuli. This method describes the technique of applying dynamic compressive strains to chondrocytes encapsulated in 3D constructs and the evaluation of induced changes in chondrocyte metabolism.

Biology

C. elegans Chemotaxis Assay

Olivia Margie¹, Chris Palmer², Ian Chin-Sang³

¹Life Sciences, Queen's University , ²Department of Chemical Engineering, Queen's University , ³Department of Biology, Queen's University

A method of quantitatively evaluating the chemotactic response of Caenorhabditis elegans is described. A chemotactic index (CI) was employed as a way to precisely evaluate the response of worms to certain targets, and serve as a platform of comparison between strains and compounds of interest.