JoVE Logo
Faculty Resource Center

Sign In

Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur

5 ARTICLES PUBLISHED IN JoVE

image

Advanced Biology

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
Perchet Thibaut 1,2,3, Sophie1 Novault 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

image

Advanced Biology

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

image

Advanced Biology

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
Perchet Thibaut 1,2,3, Meunier Sylvain 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platform, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

image

Advanced Biology

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

image

Genetics

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Thibaut Perchet 1, Sylvestre Chea 1, Milena Hasan 2, Ana Cumano 1, Rachel Golub 1
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20

This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved