Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur
5 ARTICLES PUBLISHED IN JoVE
Advanced Biology
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
Perchet Thibaut 1,2,3, Sophie1 Novault 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
Advanced Biology
Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute
Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
Advanced Biology
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
Perchet Thibaut 1,2,3, Meunier Sylvain 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platform, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
Advanced Biology
Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute
Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
Genetics
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Thibaut Perchet 1, Sylvestre Chea 1, Milena Hasan 2, Ana Cumano 1, Rachel Golub 1
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20
This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.
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