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Charité University Medicine Berlin

8 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Electrode Positioning and Montage in Transcranial Direct Current Stimulation
Alexandre F. DaSilva 1, Magdalena Sarah Volz 2,3, Marom Bikson 4, Felipe Fregni 2
1Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Material Sciences, School of Dentistry, University of Michigan , 2Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 3Charité, University Medicine Berlin, 4Department of Biomedical Engineering, The City College of New York

Transcranial direct current stimulation (tDCS) is an established technique to modulate cortical excitability1,2. It has been used as an investigative tool in neuroscience due to its effects on cortical plasticity, easy operation, and safe profile. One area that tDCS has been showing encouraging results is pain alleviation 3-5.

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Medicine

Technique and Considerations in the Use of 4x1 Ring High-definition Transcranial Direct Current Stimulation (HD-tDCS)
Mauricio F. Villamar 1,2, Magdalena Sarah Volz 1,3, Marom Bikson 4, Abhishek Datta 1,4, Alexandre F. DaSilva *5, Felipe Fregni *1
1Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 2School of Medicine, Pontifical Catholic University of Ecuador, 3Charité University Medicine Berlin, 4The City College of The City University of New York, 5Headache & Orofacial Pain Effort (H.O.P.E.), Biologic & Materials Sciences, School of Dentistry, University of Michigan

High-definition transcranial direct current stimulation (HD-tDCS), with its 4x1-ring montage, is a noninvasive brain stimulation technique that combines both the neuromodulatory effects of conventional tDCS with increased focality. This article provides a systematic demonstration of the use of 4x1 HD-tDCS, and the considerations needed for safe and effective stimulation.

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Biology

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Dörte Faust 1, Andrea Geelhaar 1, Beate Eisermann 1, Jenny Eichhorst 2, Burkhard Wiesner 2, Walter Rosenthal 1,3, Enno Klussmann 1
1Anchored Signalling, Max-Delbrück-Center for Molecular Medicine, 2Leibniz Institute for Molecular Pharmacology (FMP), 3Charité University Medicine Berlin

Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.

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Medicine

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats
Sarah Jeuthe 1, Darach O H-Ici 1, Ulrich Kemnitz 1, Thore Dietrich 2, Bernhard Schnackenburg 3, Felix Berger 1, Titus Kuehne 1, Daniel Messroghli 1,2
1Congenital Heart Disease and Pediatric Cardiology, German Heart Institute Berlin, 2Internal Medicine - Cardiology, German Heart Institute Berlin, 3Philips Health Care

Contrast enhanced cardiac magnetic resonance (CMR) imaging allows comprehensive in vivo assessment of the heart in small animal models of cardiovascular disease. Here we detail the procedures to perform CMR imaging, reconstruction and analysis using Small Animal Lock-Locker Inversion Recovery (SALLI) in rats.

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Biology

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
Victoria Kegel *1, Daniela Deharde *1, Elisa Pfeiffer 1, Katrin Zeilinger 2, Daniel Seehofer 1, Georg Damm 1
1Department of General-, Visceral- and Transplantation Surgery, Charité University Medicine Berlin, 2Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin

A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.

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Neuroscience

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats
Jens K. Haumesser 1, Johanna Kühn 1, Christopher Güttler 1, Dieu-Huong Nguyen 1, Maximilian H. Beck 1, Andrea A. Kühn 1, Christoph van Riesen 1
1Department of Neurology, Movement Disorder and Neuromodulation Unit Berlin, Charité University Medicine Berlin

In this study, the methodology is presented on how to perform multi-site in vivo electrophysiological recordings from the hyperdirect pathway under urethane anesthesia.

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Medicine

Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement
Xiaolin Sun *1,2, Yimeng Hao *1,2, Jonathan Frederik Sebastian Kiekenap 1, Jasper Emeis 1, Marvin Steitz 2, Alexander Breitenstein-Attach 2, Felix Berger 1,2, Boris Schmitt 1,2,3
1Department of Pediatric Cardiology and Congenital Heart Disease, Charité University Medicine Berlin, 2Department of Pediatric Cardiology and Congenital Heart Disease, Deutsches Herzzentrum Berlin, 3DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research)

This study assessed a new methodology with a straightened model generated from the four-dimensional cardiac computed tomography sequence to obtain the desired measurements for valve sizing in the application of transcatheter pulmonary valve replacement.

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Medicine

Transcatheter Pulmonary Valve Replacement from Autologous Pericardium with a Self-Expandable Nitinol Stent in an Adult Sheep Model
Yimeng Hao 1,2, Xiaolin Sun 1,2, Jonathan Frederik Sebastian Kiekenap 1, Jasper Emeis 1, Marvin Steitz 2, Alexander Breitenstein-Attach 2, Felix Berger 1,2, Boris Schmitt 1,2,3,4,5
1Department of Pediatric Cardiology and Congenital Heart Disease, Charité University Medicine Berlin, 2Department of Pediatric Cardiology and Congenital Heart Disease, Deutsches Herzzentrum Berlin, 3DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research), 4BIH (Berlin Institute of Health), 5BCRT (BIH Center of Regenerative Therapies)

This study demonstrates the feasibility and safety of developing an autologous pulmonary valve for implantation at the native pulmonary valve position by using a self-expandable Nitinol stent in an adult sheep model. This is a step toward developing transcatheter pulmonary valve replacement for patients with right ventricular outflow tract dysfunction.

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