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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

36.5K Views

08:47 min

March 30th, 2016

DOI :

10.3791/53069-v

March 30th, 2016


Transcript

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Primary Human Hepatocytes

Chapters in this video

0:05

Title

0:33

Perfusion and Digestion of Liver Tissue Sample

2:36

Isolation of Hepatocytes

3:56

Isolation of Non-parenchymal Liver Cells

5:16

Separation of Kupffer Cells, Endothelial Cells, and Stellate Cells

7:07

Results: Morphology of Human Parenchymal and Non-parenchymal Liver Cells in Suspension and After Adherence

7:57

Conclusion

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