Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970

1 ARTICLES PUBLISHED IN JoVE

Medicine

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

Victor Fitoussi¹, Nathalie Faye¹, Foucauld Chamming's^1,2, Olivier Clement^1,2, Charles-Andre Cuenod^1,2, Laure S. Fournier^1,2

¹Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, ²Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Service de Radiologie

In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.