CNRS/Université Paris Diderot

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Iris Marangon^*1, Nicole Boggetto^*2, Cécilia Ménard-Moyon³, Nathalie Luciani¹, Claire Wilhelm¹, Alberto Bianco³, Florence Gazeau¹

¹Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, ²ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, ³Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

Bioengineering

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Aurore Van de Walle¹, Claire Wilhelm¹, Nathalie Luciani¹

¹Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057 CNRS and University Paris Diderot

Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix production.

Bioengineering

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Aurore Van de Walle¹, Anouchka Plan Sangnier^1,2, Alexandre Fromain¹, Claire Wilhelm¹, Yoann Lalatonne^2,3

¹Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University Paris Diderot, ²Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science, LVTS, INSERM, UMR 1148, ³Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).