Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

4.1K Views

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08:13 min

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February 27th, 2021

DOI :

10.3791/61106-v

February 27th, 2021

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Aurore Van de Walle¹, Anouchka Plan Sangnier¹^,², Alexandre Fromain¹, Claire Wilhelm¹, Yoann Lalatonne²^,³

¹Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University Paris Diderot, ²Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science, LVTS, INSERM, UMR 1148, ³Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

Summary

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Magnetometry

Magnetic Nanoparticles

Cellular Incorporation

Biodegradation

Chemically Synthesized

Biomedical Applications

Contamination Prevention

Iron Oxide Nanoparticles

Nanoparticle Synthesis

Neodymium Magnet

Hydrochloric Acid

Human Mesenchymal Stem Cells

Culture Conditions

Coating Molecules

PH Adjustment

Chapters in this video

0:05

Introduction

0:58

Synthesis of Magnetic Nanoparticles

2:37

Culture and Magnetic Labeling of Stem Cells

3:33

Formation of Stem Cell-Spheroids

4:51

Quantification of Magnetic Nanoparticles Using a Vibrating Sample Magnetometer (VSM)

5:46

Results: VSM Measures Uptake and Biodegradation of Nanoparticles

7:22

Conclusion

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