In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosaAlice Verchère 1, Manuela Dezi 1, Isabelle Broutin 1, Martin Picard 1
1Laboratoire de Cristallographie & RMN Biologiques, CNRS & Université Paris Descartes
We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.
