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In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

11.8K Views

13:40 min

February 17th, 2014

DOI :

10.3791/50894-v

February 17th, 2014


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Membrane Protein

Chapters in this video

0:05

Title

3:53

Protein Production and Purification

5:24

Preparation of Proteoliposomes

8:35

Assessment of the Reconstitution Efficacy on Sucrose Gradient

9:37

Fluorescence Staining

10:28

Monitoring Pyranine Fluorescence as a Function of Time While a Proton Gradient is Generated

12:40

Conclusion

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