3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

9.0K Views

09:24 min

September 13th, 2018

DOI :10.3791/58345-v

September 13th, 2018

Transcript

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Keywords 3D Cell Culture

Chapters in this video

0:04

Title

1:03

Growing Cancer Spheroids

2:24

Preparing the 3D Culture Chamber

3:50

Live Cell Time-lapse Imaging

4:24

Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning

5:36

Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids

7:18

Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors

8:45

Conclusion

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