Heinrich-Heine-University Dusseldorf

2 ARTICLES PUBLISHED IN JoVE

Biology

An Innovative Method for Exosome Quantification and Size Measurement

Arash Mehdiani¹, Anatol Maier¹, Antonio Pinto¹, Mareike Barth¹, Payam Akhyari¹, Artur Lichtenberg¹

¹Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf

A method for isolation of exosomes from whole blood and further analysis by nanoparticle tracking using a semi-automatic instrument is presented in this article. The presented technology provides an extremely sensitive method for visualizing and analyzing particles in liquid suspension.

Biochemistry

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Andreas Weber¹, Julia Christin Wehmeyer¹, Vera Schmidt¹, Artur Lichtenberg¹, Payam Akhyari¹

¹Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University

In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.