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RMIT University

6 ARTICLES PUBLISHED IN JoVE

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JoVE Core

Behavioral Determination of Stimulus Pair Discrimination of Auditory Acoustic and Electrical Stimuli Using a Classical Conditioning and Heart-rate Approach
Simeon J. Morgan 1, Antonio G. Paolini 1
1School of Psychological Science, La Trobe University

The application of a classical fear conditioning behavioral paradigm for auditory prosthetic research in rats is described. This paradigm provides a mechanism for identifying both detection of, and discrimination between, distinct acoustic and electrical stimuli using heart-rate as an outcome measure.

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Neuroscience

A Method for Systematic Electrochemical and Electrophysiological Evaluation of Neural Recording Electrodes
Alexander R. Harris 1,3, Simeon J. Morgan 1,3, Gordon G. Wallace 3, Antonio G. Paolini 1,3,4
1School of Psychological Science, La Trobe University, 2Intelligent Polymer Research Institute, University of Wollongong, 3ARC Centre of Excellence for Electromaterials Science, 4Health Innovations Research Institute, College of Science, Engineering, and Health, RMIT University

Different electrode coatings affect neural recording performance through changes to electrochemical, chemical and mechanical properties. Comparison of electrodes in vitro is relatively simple, however comparison of in vivo response is typically complicated by variations in electrode/neuron distance and between animals. This article provides a robust method to compare neural recording electrodes.

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Engineering

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight
Vi Khanh Truong 1, Miljan Stefanovic 1, Shane Maclaughlin 2, Mark Tobin 3, Jitraporn Vongsvivut 3, Mohammad Al Kobaisi 1, Russell J. Crawford 4, Elena P. Ivanova 1
1School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 2BlueScope Steel Research, 3Infrared Microspectroscopy Beamline, Australian Synchrotron, 4School of Science, College of Science, Engineering and Health, RMIT University

Two types of surfaces, polyester-coated steel and polyester coated with a layer of silica nanoparticles, were studied. Both surfaces were exposed to sunlight, which was found to cause substantial changes in the chemistry and nanoscale topography of the surface.

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Immunology and Infection

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
Thomas A. Angelovich 1,2, Anna C. Hearps 1,3, Anna Maisa 1, Theodoros Kelesidis 4, Anthony Jaworowski 1,3
1Centre for Biomedical Research, Burnet Institute, 2School of Health and Biomedical Sciences, RMIT University, 3Department of Infectious Diseases, Monash University, 4University of California, Los Angeles

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

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Immunology and Infection

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Shubhendu Sen Roy 1, Huy Cong Xuan Nguyen 1, Thomas A. Angelovich 2,3, Anna C. Hearps 2, Diana Huynh 1,4, Anthony Jaworowski 2,4, Theodoros Kelesidis 1
1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University

We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

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Engineering

In Situ Transmission Electron Microscopy with Biasing and Fabrication of Asymmetric Crossbars Based on Mixed-Phased a-VOx
Shruti Nirantar *1, Edwin Mayes *2, Sharath Sriram 1
1Functional Materials and Microsystems Research Group and the Micro Nano Research Facility, RMIT University, 2RMIT Microscopy and Microanalysis Facility, RMIT University

Presented here is a protocol for analyzing nanostructural changes during in situ biasing with transmission electron microscopy (TEM) for a stacked metal-insulator-metal structure. It has significant applications in resistive switching crossbars for the next generation of programmable logic circuits and neuromimicking hardware, to reveal their underlying operation mechanisms and practical applicability.

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