Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix AssaysF. Mark Dunning 1, Timothy M. Piazza 1, Füsûn N. Zeytin 1, Ward C. Tucker 1
1BioSentinel Inc., Madison, WI
The BoTest Matrix botulinum neurotoxin (BoNT) detection assays rapidly purify and quantify BoNT from a range of sample matrices. Here, we present a protocol for the detection and quantification of BoNT from both solid and liquid matrices and demonstrate the assay with BOTOX, tomatoes, and milk.
