Université Paris Diderot, Sorbonne Paris Cité

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Olivier Etienne^1,2,3,4, Amandine Bery^1,2,3,4, Telma Roque^1,2,3,4, Chantal Desmaze^1,2,3,4, François D. Boussin^1,2,3,4

¹Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, ²INSERM, U967, ³Université Paris Diderot, Sorbonne Paris Cité, ⁴Université Paris Sud, UMR 967

Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.

Developmental Biology

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Laura Gouder^1,2,3, Jean-Yves Tinevez⁴, Hany Goubran-Botros^1,2,3, Alexandra Benchoua⁵, Thomas Bourgeron^1,2,3, Isabelle Cloëz-Tayarani^1,2,3

¹Human Genetics and Cognitive Functions, Institut Pasteur, ²CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, ³Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, ⁴Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, ⁵Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

Neuroscience

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Mathieu Daynac^*1,2,3,4,5, Lise Morizur^*1,2,3,4, Thierry Kortulewski^1,2,3,4, Laurent R. Gauthier^1,2,3,4, Martial Ruat⁵, Marc-André Mouthon^1,2,3,4, François D. Boussin^1,2,3,4

¹CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, ²INSERM, UMR 967, ³Université Paris Diderot, Sorbonne Paris Cité, UMR 967, ⁴Université Paris Sud, UMR 967, ⁵CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

Behavior

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication

Allain-Thibeault Ferhat¹, Nicolas Torquet², Anne-Marie Le Sourd¹, Fabrice de Chaumont³, Jean-Christophe Olivo-Marin³, Philippe Faure², Thomas Bourgeron¹, Elodie Ey¹

¹Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, ²Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, ³Bio Image Analysis, CNRS URA 2582, Institut Pasteur

Mouse ultrasonic vocalizations are used as proxies to model the genetic bases of vocal communication deficits in mouse models for neuropsychiatric disorders. The present protocol describes three experimental contexts that reliably elicit ultrasonic vocalizations from pups (throughout development) and adult mice (same-sex interactions, male-estrus female interactions).