Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

14.2K Views

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09:27 min

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September 14th, 2015

DOI :

10.3791/53247-v

September 14th, 2015

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Mathieu Daynac*¹^,²^,³^,⁴^,⁵, Lise Morizur*¹^,²^,³^,⁴, Thierry Kortulewski¹^,²^,³^,⁴, Laurent R. Gauthier¹^,²^,³^,⁴, Martial Ruat⁵, Marc-André Mouthon¹^,²^,³^,⁴, François D. Boussin¹^,²^,³^,⁴

¹CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, ²INSERM, UMR 967, ³Université Paris Diderot, Sorbonne Paris Cité, UMR 967, ⁴Université Paris Sud, UMR 967, ⁵CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

Summary

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Keywords Neural Stem Cells

Subventricular Zone

Fluorescence activated Cell Sorting FACS

Cell Cycle Dynamics

Transit Amplifying Cells

Chapters in this video

0:05

Title

1:38

Basic Setup

2:50

SVZ Tissue Dissociation

4:19

Immunofluorescent Staining and Cell Sorting

6:21

Microscope Setup and Image Acquisition

7:57

Results: Live Imaging of the Cell Cycle Phases at the Level of a Single Cell

8:53

Conclusion

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