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Université Paris Diderot

6 ARTICLES PUBLISHED IN JoVE

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Biology

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
Alexis Peaucelle 1,2
1Laboratoire Matière et Systèmes Complexes, UFR de Physique, Université Paris Diderot, 2Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon

We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.

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Chemistry

High Precision Zinc Isotopic Measurements Applied to Mouse Organs
Frédéric Moynier 1, Marie Le Borgne 2
1Institut de Physique du Globe de Paris, Institut Universitaire de France, Université Paris Diderot, 2INSERM (Institut National de la Santé et de la Recherche Médicale), U1148: Laboratory for Vascular Translational Science, Université Paris Diderot

We present the technique to measure with high precision zinc isotope ratios in mouse organs.

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Medicine

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis
Mathieu Manassero 1,2, Adeline Decambron 1,2, Bui Truong Huu Thong 1, Véronique Viateau 1,2, Morad Bensidhoum 1, Hervé Petite 1
1Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires (B2OA - UMR CNRS 7052), Université Paris Diderot, 2Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est

Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity.

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Neuroscience

Purification of Mouse Brain Vessels
Anne-Cécile Boulay 1,2,3, Bruno Saubaméa 4, Xavier Declèves 4, Martine Cohen-Salmon 1,2,3
1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241, Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie, Université Paris Diderot

We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.

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Medicine

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models
Jeremie Cosette 1,2,3, Rym Ben Abdelwahed 2, Sabrina Donnou-Triffault 1,4,5, Catherine Sautès-Fridman 2, Patrice Flaud *3, Sylvain Fisson *1,4,5
1Genethon, 2Cordeliers Research Center, INSERM U872, 3Matter and Complex Systems Laboratory, Université Paris Diderot, 4INSERM U951, 5UMR_S951, Université d'Evry Val d'Essonne

Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.

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Chemistry

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
Caroline Bissardon 1, Marie-Pierre Isaure 2, Emmanuel Lesuisse 3, Mauro Rovezzi 4,5, Eric Lahera 4,5, Olivier Proux 4,5, Sylvain Bohic 1,6
1University Grenoble Alpes, INSERM UA7, Synchrotron Radiation for Biomedicine (STROBE), 2CNRS, UMR 5254, Université Pau & Pays Adour, E2S UPPA, Institut des Sciences Analytiques et de Physicochimie pour l'Environnement et les Matériaux, 3Institut Jacques Monod, UMR 7592 CNRS, Université Paris Diderot, 4OSUG, UMS 832 CNRS, Université Grenoble Alpes, 5FAME and FAME-UHD beamlines, ESRF, the European Synchrotron, 6ID16A beamline, ESRF, the European Synchrotron

This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample cryo-preparation.

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