VU University Amsterdam

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons

Rajeevan T. Narayanan^*1, Hemanth Mohan^*1, Robin Broersen^*1, Roel de Haan¹, Anton W. Pieneman¹, Christiaan P.J. de Kock¹

¹Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam

To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.

Biology

In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae

Alberto Natali¹, Laura M. Roy¹, Roberta Croce¹

¹Department of Physics and Astronomy, VU University Amsterdam

This protocol details the reconstitution of light-harvesting complexes in vitro. These integral membrane proteins coordinate chlorophylls and carotenoids and are responsible for harvesting light in higher plants and green algae.