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Genentech, Inc.

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Ali Ertürk 1, Daniel Lafkas 2, Cecile Chalouni 3
1Neuroscience, Genentech, Inc., 2Department of Discovery Oncology, Genentech, Inc., 3Department of Pathology, Genentech, Inc.

To obtain high-resolution images of fluorescently labeled cells within large tissues, ideally, the biological samples should be imaged without sectioning. 3DISCO is a straightforward tissue clearing procedure based on sequential incubation with organic solvents. Upon clearing, the organs become transparent allowing an end-to-end laser scan of the specimen.

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Chemistry

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Maria Victoria Silva Elipe 1, Lan Li 1, Karthik Nagapudi 2, Alan M. Kook 3, Carlos Cobas 4, Isaac Iglesias 4, Chen Peng 4
1Department of Attribute Sciences, Amgen, Inc., 2Small Molecule Pharmaceutical Sciences, Genentech, Inc., 3NMR Service + Consulting, 4Mestrelab Research

A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry.

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Cancer Research

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
Charles A. Havnar 1, Oliver Zill 2, Jeff Eastham 1, Jeffrey Hung 1, Manana Javey 3, Emmanuel Naouri 3, Jennifer Giltnane 1, Justin M. Balko 4, Andrew Wallace 2, Nicolas Lounsbury 2, Daniel Oreper 2, Sarajane Saturnio 1, G-Y Yang 5, Amy A. Lo 1
1Departments of Research Pathology, Genentech, 2Bioinformatics & Computational Biology, Genentech, 3Roche Sequencing Solutions, Hacienda Drive, 4Department of Medicine, Vanderbilt University Medical Center, Medical Center Drive, 5Department of Pathology, Northwestern University, Feinberg School of Medicine

Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.

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Medicine

Synthetic Antigen Controls for Immunohistochemistry
Charles A. Havnar 1, Kathy J. Hötzel 1, Charles A. Jones 1, Carmina M. Espiritu 1, Linda K. Rangell 1, Franklin V. Peale 1
1Research Pathology, Genentech, Inc.

This work documents a simple method to create synthetic antigen controls for immunohistochemistry. The technique is adaptable to a variety of antigens in a wide range of concentrations. The samples provide a reference with which to assess intra- and inter-assay performance and reproducibility.

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