Pilot Evaluation of the Peptide Dilution Series and BSA Gel Tissue Microarray Construction
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Results: Staining of ERBB2/HER2 Peptide TMA with ERBB2/HER2 1+ and 3+ Cell Lines
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Conclusion
Transcript
BSA antigen gels are well-characterized reproducible IHC standards that can supplement existing controls. This protocol uses standard histology and IHC reagents and methods to make reproducible standards of known composition. These controls provid
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This work documents a simple method to create synthetic antigen controls for immunohistochemistry. The technique is adaptable to a variety of antigens in a wide range of concentrations. The samples provide a reference with which to assess intra- and inter-assay performance and reproducibility.