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Philipps-Universität Marburg

4 ARTICLES PUBLISHED IN JoVE

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Biology

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
Stefanie M. K. Gärtner 1, Christina Rathke 1, Renate Renkawitz-Pohl *1, Stephan Awe *2
1Fachbereich Biologie, Entwicklungsbiologie, Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

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Immunology and Infection

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Sudhanshu Bhushan 1, Ferial Aslani 1, Zhengguo Zhang 1, Tim Sebastian 1, Hans-Peter Elsässer 2, Jörg Klug 1
1Institut für Anatomie und Zellbiologie, Justus-Liebig-Universität Gießen, 2Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg

Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.

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Behavior

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats
Liana Melo-Thomas 1,2, K.-Alexander Engelhardt 1, Uwe Thomas 3, Dirk Hoehl 3, Sascha Thomas 3, Markus Wöhr 1, Bjoern Werner 4, Frank Bremmer 4, Rainer K.W. Schwarting 1
1Behavioral Neuroscience, Experimental and Biological Psychology, Philipps-Universität Marburg, 2Behavioral Neurosciences Institute (INeC), 3Thomas RECORDING GmbH, 4Department of Neurophysics, Philipps-Universität Marburg

A wireless, bidirectional system for multi-channel neural recordings and stimulation in freely behaving rats is introduced. The system is light and compact, thus having minimal impact on the animal´s behavioral repertoire. Moreover, this bidirectional system provides a sophisticated tool to assess causal relationships between brain activation patterns and behavior.

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Biochemistry

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
Jan Wollenhaupt 1, Tatjana Barthel 1,2, Gustavo M. A. Lima 3, Alexander Metz 4, Dirk Wallacher 5, Elmir Jagudin 3, Franziska U. Huschmann 1,4, Thomas Hauß 1, Christian G. Feiler 1, Martin Gerlach 1, Michael Hellmig 1, Ronald Förster 1, Michael Steffien 1, Andreas Heine 4, Gerhard Klebe 4, Uwe Mueller 1, Manfred S. Weiss 1
1Macromolecular Crystallography, Helmholtz-Zentrum Berlin, 2Structural Biochemistry Group, Institute for Chemistry and Biochemistry, Freie Universität Berlin, 3BioMAX, MAX IV Laboratory, 4Drug Design Group, Institute of Pharmaceutical Chemistry, Philipps-Universität Marburg, 5Department Sample Environment, Helmholtz-Zentrum Berlin

Crystallographic fragment screening at the Helmholtz-Zentrum Berlin is performed using a workflow with dedicated compound libraries, crystal handling tools, fast data collection facilities and largely automated data analysis. The presented protocol intends to maximize the output of such experiments to provide promising starting points for downstream structure-based ligand design.

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