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Miami University

4 ARTICLES PUBLISHED IN JoVE

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Biology

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum
David M. Linz *1, Courtney M. Clark-Hachtel *1, Ferran Borràs-Castells *1, Yoshinori Tomoyasu 1
1Department of Biology, Miami University

RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.

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Medicine

Dissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling
Michelle J. Veite-Schmahl 1, Daniel P. Regan 1, Adam C. Rivers 1, Joseph F. Nowatzke 1,2, Michael A. Kennedy 1
1Department of Chemistry & Biochemistry, Miami University, 2College of Medicine, Central Michigan University

This video article provides a detailed demonstration of the procedures required to successfully remove the pancreas from a mouse by dissection for histological analysis and metabolic profiling.

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JoVE Journal

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
Shubham Rathore 1, Jenni Hassert 1, Courtney M. Clark-Hachtel 2,3, Aaron Stahl 1,4, Yoshinori Tomoyasu 2, Elke K. Bushbeck 1
1Department of Biological Sciences, University of Cincinnati, 2Department of Biology, Miami University, 3Department of Biology, University of North Carolina at Chapel Hill, 4Department of Neuroscience, The Scripps Research Institute

RNA interference is a widely applicable, powerful technique for manipulating gene expression at specific developmental stages. Here, we describe the necessary steps for implementing this technique in the aquatic diving beetle Thermonectus marmoratus, from the acquisition of gene sequences to the knockdown of genes that affect structure or behavior.

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Engineering

Measurement of the Hand Transmitted Vibration of the Human Hand Arm System During Operation of a Hand Tractor
Shiqing Lu 1, Rui Jiang 2, Xinyi Xiao 3, Yong Li 1, Xia Huang 1, Kun Song 1, Changming Chen 1, Jun Ding 1
1School of Mechanical Engineering, Chongqing University of Technology, 2College of Mechanical and Electrical Engineering, Wenzhou University, 3Mechanical and Manufacturing Engineering, Miami University

Here, we present a standardized method for measurement of the hand transmitted vibration from handles of a single-axle tractor with special reference to changes in grip force and vibration frequency.

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