RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

7.6K Views

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08:55 min

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May 29th, 2020

DOI :

10.3791/61477-v

May 29th, 2020

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Shubham Rathore¹, Jenni Hassert¹, Courtney M. Clark-Hachtel²^,³, Aaron Stahl¹^,⁴, Yoshinori Tomoyasu², Elke K. Bushbeck¹

¹Department of Biological Sciences, University of Cincinnati, ²Department of Biology, Miami University, ³Department of Biology, University of North Carolina at Chapel Hill, ⁴Department of Neuroscience, The Scripps Research Institute

Summary

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Microinjection Technique

Stereo Microscope

Embryonic Manipulation

Injection Pressure

Chapters in this video

0:04

Introduction

0:37

Early stage T. marmoratus Embryo Preparation

1:58

Early Stage T. marmoratus Embryo Double Stranded RNA (dsRNA) Microinjection

5:04

T. marmoratus Larva Preparation

5:58

T. marmoratus Larva dsRNA Microinjection

7:05

Results: Representative Effects of RNA Interference in Aquatic Beetles at Different Developmental Time Points

8:18

Conclusion

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