Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80Kentaro Ohkuni 1, Yoshimitsu Takahashi 1, Munira A. Basrai 1
1Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health
This manuscript describes the detection of sumoylation and ubiquitination of kinetochore proteins, Ndc10 and Ndc80, in the budding yeast Saccharomyces cerevisiae.
