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National Cancer Institute, National Institute of Health

1 ARTICLES PUBLISHED IN JoVE

Biology

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Kentaro Ohkuni¹, Yoshimitsu Takahashi¹, Munira A. Basrai¹

¹Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health

This manuscript describes the detection of sumoylation and ubiquitination of kinetochore proteins, Ndc10 and Ndc80, in the budding yeast Saccharomyces cerevisiae.