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Xenopus laevis as a Model to Identify Translation Impairment

DOI :

10.3791/52724-v

September 27th, 2015

September 27th, 2015

10,574 Views

1Team "Early Stages of Parkinson's Disease" of the Jean-Pierre Aubert Research Center, INSERM UMR-S1172, CHRU Lille, University of Lille 1 and Lille 2, 2Team "Signal Division Regulation", CNRS UMR 8576, University of Lille 1

Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.

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Keywords Xenopus Laevis

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