Institut du cancer de Montréal

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Antonio Layoun¹, Macha Samba¹, Manuela M. Santos^1,2

¹Centre de recherche du Centre hospitalier de l'Université de Montréal, Institut du cancer de Montréal, ²Département de Médecine, Université de Montréal

We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

Immunology and Infection

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Manuel Flores Molina^1,2, Thomas Fabre^1,2, Aurélie Cleret-Buhot¹, Geneviève Soucy^1,3, Liliane Meunier^1,4, Mohamed N. Abdelnabi^1,2, Nicolas Belforte^1,5, Simon Turcotte^1,4,6, Naglaa H. Shoukry^1,4,7

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, ³Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, ⁴Institut du Cancer de Montréal, ⁵Département de Neurosciences, Faculté de Médecine, Université de Montréal, ⁶Département de Chirurgie, Faculté de Médecine, Université de Montréal, ⁷Département de Médecine, Faculté de médecine, Université de Montréal

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

Biology

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

Claire Gerkins¹, Roy Hajjar^1,2, Manon Oliero¹, Manuela M. Santos^1,3

¹Nutrition and Microbiome Laboratory, Institut du cancer de Montréal, Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), ²Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine, Université de Montréal, ³Department of Medicine, Faculty of Medicine, Université de Montréal

In the present study, fluorescein-isothiocyanate-labeled (FITC) dextran is administered to mice via oral gavage to evaluate intestinal permeability both in vivo and in plasma and fecal samples. As gut barrier function is affected in many disease processes, this direct and quantitative assay can be used in diverse research areas.