Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
17.1K Views
•
11:00 min
•
March 25th, 2020
DOI :
March 25th, 2020
•Manuel Flores Molina1,2, Thomas Fabre1,2, Aurélie Cleret-Buhot1, Geneviève Soucy1,3, Liliane Meunier1,4, Mohamed N. Abdelnabi1,2, Nicolas Belforte1,5, Simon Turcotte1,4,6, Naglaa H. Shoukry1,4,7
1Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), 2Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, 3Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, 4Institut du Cancer de Montréal, 5Département de Neurosciences, Faculté de Médecine, Université de Montréal, 6Département de Chirurgie, Faculté de Médecine, Université de Montréal, 7Département de Médecine, Faculté de médecine, Université de Montréal
Chapters in this video
0:05
Introduction
1:03
Heat-Induced Antigen Retrieval
1:46
Nonspecific Binding Blocking
2:28
Immunofluorescence Labeling
3:36
Image Alignment
4:36
Tissue Detection
5:35
Tissue Segmentation
6:28
Cell of Interest (COI) Identification and Quantification
7:15
Tissue Heatmapping
8:10
Results: Representative Visualization, Quantification, and Mapping of Cell Populations of Interest in the Tumor Microenvironment
10:02
Conclusion
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