Charité – Universitätsmedizin Berlin

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Orthotopic Hind-Limb Transplantation in Rats

Robert Sucher^*1, Rupert Oberhuber^*1, Christian Margreiter¹, Guido Rumberg¹, Rishi Jindal², WP Andrew Lee², Raimund Margreiter¹, Johann Pratschke¹, Stefan Schneeberger¹, Gerald Brandacher¹

¹Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, ²Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center

Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.

Medicine

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Rupert Oberhuber^*1, Benno Cardini^*1, Markus Kofler¹, Paul Ritschl¹, Robert Oellinger¹, Felix Aigner¹, Robert Sucher¹, Stefan Schneeberger¹, Johann Pratschke¹, Gerald Brandacher², Manuel Maglione¹

¹Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, ²Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine

The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach.

Herein, we provide additional details of this technique to supplement the video.

Bioengineering

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Karl Hillebrandt¹, Dietrich Polenz¹, Antje Butter¹, Peter Tang¹, Anja Reutzel-Selke¹, Andreas Andreou¹, Hendrik Napierala¹, Nathanael Raschzok¹, Johann Pratschke¹, Igor M. Sauer¹, Benjamin Struecker¹

¹General, Visceral, and Transplantation Surgery, Charité – Universitätsmedizin Berlin

The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.

Neuroscience

Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy

Paul V. Ritschl^*1,2,3, Lena Hofhansel^*2,4, Bernhard Flörchinger⁵, Rupert Oberhuber², Robert Öllinger¹, Johann Pratschke¹, Katja Kotsch⁶

¹Department of Surgery Campus Charité Mitte/Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, ²Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, ³Charité Clinician Scientist Program, Berlin Institute of Health, ⁴Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, ⁵Department of Cardiothoracic Surgery, University Medical Center Regensburg, ⁶Department of General, Visceral and Vascular Surgery, Charité - Universitätsmedizin Berlin

We present a murine model of brain death induction in order to evaluate the influence of its pathophysiological effects on organs as well as on consecutive grafts in the context of solid organ transplantation.

Immunology and Infection

Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples

Dario L. Frey^*1,2, Matteo Guerra^*1,2,3,4, Marcus A. Mall^1,2,5,6,7, Carsten Schultz^1,3,8

¹Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), ²Dept. of Translational Pulmonology, University of Heidelberg, ³Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, ⁴Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, ⁵Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, ⁶Berlin Institute of Health (BIH), ⁷German Center for Lung Research (DZL), Associated Partner Site, Berlin, ⁸Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University

The protocols herein described provide a guide to visualize and quantify the activity of neutrophil proteases in human sputum. The applications of such analysis span from the evaluation of anti-inflammatory treatments, to biomarker validation, drug screening and large cohort clinical studies.