Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples
15.6K Views
•
09:23 min
•
May 21st, 2021
DOI :
May 21st, 2021
•1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
Chapters in this video
0:05
Introduction
1:07
Cell Isolation and Supernatant Separation
2:36
Quantification of Soluble Neutrophil Serine Protease (NSP) Activity via Fluorimeter or Plate Reader Assay
4:13
Membrane-bound NSPs Activity Measurement via Fluorescence Microscopy
5:30
Membrane-bound NSPs Activity Measurement via Flow Cytometry
7:25
Results: Representative Images and Quantification of Membrane-Bound NE Activity on Neutrophils Isolated from CF Patient Sputum
8:26
Conclusion
Related Videos
11:03
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
13.0K Views
12:42
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
10.0K Views
10:59
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
905 Views
07:37
Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
6.3K Views
13:28
Methodology for Sputum Induction and Laboratory Processing
27.4K Views
14:05
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
2.3K Views
07:18
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
3.2K Views
07:19
Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils
898 Views
11:32
Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists
511 Views
01:23
An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter
64 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved