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Lausanne University Hospital

4 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films
Ruslan Cusnir 1, Philipp Steinmann 2, Marcus Christl 3, François Bochud 1, Pascal Froidevaux 1
1Institute of Radiation Physics, Lausanne University Hospital, 2Federal Office of Public Health, Bern, Switzerland, 3Laboratory of Ion Beam Physics, ETH Zurich

The technique of diffusive gradients in thin films (DGT) is proposed for speciation studies of plutonium. This protocol describes diffusion experiments probing the behavior of Pu(IV) and Pu(V) in presence of organic matter. DGTs deployed in a karstic spring allow assessment of the bioavailability of Pu.

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Behavior

Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice
Jessica Lavier 1,2, Manon Beaumann 3, Steeve Ménetrey 3, Lucia Mazzolai 1, Anne-Christine Peyter 3, Maxime Pellegrin 1, Grégoire P. Millet 2
1Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), 2Institute of Sport Sciences, Faculty of Biology and Medicine, University of Lausanne, 3Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital

High-intensity training in hypoxia is a protocol that has been proven to induce vascular adaptations potentially beneficial in some patients and to improve athletes' repeated sprint ability. Here, we test the feasibility of training mice using that protocol and identify those vascular adaptations using ex vivo vascular function assessment.

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Genetics

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
Antonio Mancarella 1, Francesco A. Procopio 1, Tilmann Achsel 2, Elisa De Crignis 3, Brian T. Foley 4, Giampietro Corradin 5, Claudia Bagni 2, Giuseppe Pantaleo 1, Cecilia Graziosi 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.

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Medicine

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
Martino Guiotto *1,4, Silvia Palombella *2, Stefania Brambilla 2, Lee Ann Applegate 3, Mathis Riehle 4, Andrew Hart 4, Wassim Raffoul 1, Pietro Giovanni di Summa 1
1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2Cell and Tissue Engineering Lab, IRCCS Ospedale Galeazzi Sant'Ambrogio, 3Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine, Lausanne University Hospital, 4Centre for the Cellular Microenvironment, University of Glasgow

Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.

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