A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNAHemant Suryawanshi *1, Mayuresh Anant Sarangdhar *1,2, Manika Vij 1,2, Reema Roshan 1, Vijay Pal Singh 1, Munia Ganguli 1, Beena Pillai 1,2
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India
MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.
