<p class="jove_content">Our overall aim is to understand how cells sense extracellular cues that lead to directed axonal growth. Here, we describe the methodology of Light-Induced Molecular Adsorption of Proteins, used to produce defined micro-patterns of extracellular matrix components in order to study specific events that govern axon outgrowth and pathfinding.</p>

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Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Cells sense a variety of extracellular cues, including the composition and geometry of the extracellular matrix, which is synthesized and remodeled by the ...

<p class='jove_content'>Identifying proteins specifically associated with Bruch&#8217;s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.</p>

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Enrichment of Bruch's Membrane from Human Donor Eyes

Age-related macular degeneration (AMD) is a leading cause of visual impairment in the developed world. The disease manifests itself by the destruction of ...

Centre for Advanced Discovery and Experimental Therapeutics

Central Manchester University Hospitals NHS Foundation Trust

Blond McIndoe Laboratories

School of Biological Sciences

Faculty of Biology

Medicine and Health

Division of Cell Matrix Biology and Regenerative Medicine

The University of Manchester

Department of Plastic Surgery & Burns

Wythenshawe Hospital

Manchester University NHS Foundation Trust

Centre for Advanced Discovery and Experimental Therapeutics, Central Manchester University Hospitals NHS Foundation Trust

Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester

Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust

Manchester Academic Health Science Centre

Institution - Manchester Academic Health Science Centre

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