Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

11.9K Views

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09:49 min

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October 11th, 2019

DOI :

10.3791/60092-v

October 11th, 2019

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Cristina Melero*¹, Aljona Kolmogorova*¹, Paul Atherton¹, Brian Derby², Adam Reid³^,⁴, Karin Jansen⁵, Christoph Ballestrem¹

¹Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research, The University of Manchester, ²School of Materials, The University of Manchester, ³Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Manchester Academic Health Science Centre, ⁴Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, ⁵Department of Pathology, UMC Utrecht

Summary

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Light Induced Molecular Adsorption

PRIMO System

Micro patterning

Cell Responses

Extracellular Matrix Proteins

Regenerative Medicine

PDMS Stencils

PLL PEG Solution

Cell Biology

Chapters in this video

0:04

Title

0:57

Design of Pattern Templates and Plasma Cleaning

1:52

Passivation with PLL-PEG

2:54

System Calibration

4:36

Software Set-up and Photopatterning

5:29

Protein Incubation and Blocking Unspecific Binding Sites

6:43

Second Round of Photopatterning and Protein Incubation

7:39

Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins

9:03

Conclusion

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