Oslo University Hospital

6 ARTICLES PUBLISHED IN JoVE

Biology

Xenotransplantation of Human Stem Cells into the Chicken Embryo

Jean-Luc Boulland¹, Gabor Halasi¹, Nedim Kasumacic¹, Joel C. Glover^1,2

¹Department of Physiology, University of Oslo, ²Norwegian Center for Stem Cell Research, University of Oslo

In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.

Medicine

A Neonatal Mouse Spinal Cord Compression Injury Model

Mark Züchner^1,2, Joel C. Glover^2,3, Jean-Luc Boulland^2,3

¹Department of Neurosurgery, Oslo University Hospital, ²Norwegian Center for Stem Cell Research, Oslo University Hospital, ³Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Physiology, Institute of Basic Medical Sciences, University of Oslo

This article describes a method for generating a reproducible spinal cord compression injury (SCI) in the neonatal mouse. The model provides an advantageous platform for studying mechanisms of adaptive plasticity that underlie spontaneous functional recovery.

Medicine

The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo

Ane M. Holme^1,2, Maia B. Holm^1,2, Marie C. P. Roland^1,3, Hildegunn Horne^1,2, Trond M. Michelsen^1,3, Guttorm Haugen^2,4, Tore Henriksen^1,2

¹Department of Obstetrics, Oslo University Hospital, ²Institute of Clinical Medicine, University of Oslo, ³Norwegian Advisory Unit on Women's Health, Oslo University Hospital, ⁴Department of Fetal Medicine, Oslo University Hospital

We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.

Neuroscience

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Lauritz Hagen Kennedy¹, Johanne Egge Rinholm^1,2

¹Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, ²Department of Microbiology, Oslo University Hospital

Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.

Biology

Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion

Hege Brincker Fjerdingstad¹, Joel C. Glover^1,2

¹Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, ²Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo

To avoid the limitations associated with the enzymatic or mechanical passaging of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) cultured on feeder cells, we have established a fast, effective, cost-efficient, high-yield method for harvesting hESC or hiPSC colonies maintained on a feeder cell layer of human foreskin fibroblasts using EDTA-mediated dis-adhesion.

Neuroscience

Application of Flow Cytometric Analysis for Measuring Multiple Mitochondrial Parameters in 3D Brain Organoids

Sharika Marjan^1,2, Tsering Yangzom^1,2, Cecilie Katrin Kristiansen^1,3, Anbin Chen⁴, Laurence A. Bindoff^1,5,6, Kristina Xiao Liang^1,3

¹Department of Clinical Medicine (K1), University of Bergen, ²Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, ³Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, ⁴Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, ⁵Department of Neurology, Haukeland University Hospital, ⁶National Advisory Unit for Congenital Metabolic Diseases, Oslo University Hospital

This protocol reports a unique method of using a streaming cytometer and multiple antibodies for simultaneous assessment of multiple mitochondrial functional parameters, including changes in mitochondrial volume, amounts of the mitochondrial respiratory chain (MRC) complex subunits, and mitochondrial DNA (mtDNA) replication.