Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion

521 Views

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07:21 min

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July 7th, 2023

DOI :

10.3791/63788-v

July 7th, 2023

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Hege Brincker Fjerdingstad¹, Joel C. Glover¹^,²

¹Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, ²Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo

Summary

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Human Pluripotent Stem Cells

Enzyme free Passaging

Feeder Cells

Ethylenediaminetetraacetic Acid

Cost efficient Method

Chapters in this video

0:00

Introduction

0:38

Cultivation of Human Fibroblast Cells and Preparation of the Feeder Cell Layer

3:42

Harvesting hESC or hiPSC Colonies Using EDTA‐Mediated Dis-Adhesion

5:15

Results: Comparison of EDTA‐Mediated Dis‐Adhesion and Mechanical Harvesting of hESCs Cultured on Feeder Cells

6:40

Conclusion

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