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HHMI Janelia Research Campus

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
Jaroslav Icha *1, Christopher Schmied *1, Jaydeep Sidhaye 1, Pavel Tomancak 1, Stephan Preibisch 1,2,3, Caren Norden 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Immunology and Infection

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
James J. Faust 1,2, Wayne Christenson 3,4,5, Kyle Doudrick 6, John Heddleston 7, Teng-Leong Chew 7, Marko Lampe 8, Arnat Balabiyev 1,2, Robert Ros 3,4,5, Tatiana P. Ugarova 1,2
1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

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