Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
7.3K Views
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08:50 min
•
March 14th, 2018
DOI :
March 14th, 2018
•James J. Faust1,2, Wayne Christenson3,4,5, Kyle Doudrick6, John Heddleston7, Teng-Leong Chew7, Marko Lampe8, Arnat Balabiyev1,2, Robert Ros3,4,5, Tatiana P. Ugarova1,2
1Center for Metabolic and Vascular Biology, Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences, Arizona State University, 3Department of Physics, Arizona State University, 4Center for Biological Physics, Arizona State University, 5Biodesign Institute, Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 7Advanced Imaging Center, HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility, European Molecular Biology Laboratory
Chapters in this video
0:05
Title
0:47
Preparing Acid-cleaned Cover Glass
1:59
Preparing of Fusogenic Optical-quality Surfaces
2:52
Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
3:58
Fabricating Glass Bottom Dishes
4:55
Collecting Thioglycollate-elicited Macrophages
6:15
Results: Macrophage Fusion on Glass Surfaces
7:51
Conclusion
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