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Canadian Institute for Advanced Research

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Foraging Path-length Protocol for Drosophila melanogaster Larvae
Ina Anreiter 1, Oscar E. Vasquez 1, Aaron M. Allen 2, Marla B. Sokolowski 1,3
1Department of Ecology and Evolutionary Biology, University of Toronto, 2Department of Cell and Systems Biology, University of Toronto, 3Child and Brain Development Program, Canadian Institute for Advanced Research

We provide a detailed protocol for a Drosophila melanogaster foraging path-length assay. We discuss the preparation and handling of test animals, how to perform the assay and analyze the data.

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Immunology and Infection

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Nicola Pett 1, Michael Hunter 1, Natalia A. Carranza García 1, Jung Hee Seo 1, Samuel R. Collins 1, Forest Rohwer 2, Lisa C. Osborne 1, Carolina Tropini 1,3,4
1Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, 2Department of Biology, San Diego State University, 3School of Biomedical Engineering, University of British Columbia, 4Humans and the Microbiome Program, Canadian Institute for Advanced Research

Bacteriophages (phages), viruses that infect bacteria, are an integral component of the gut microbiome. Though these symbiotic inhabitants drive bacterial fitness and population dynamics, little is understood about how they impact gut homeostasis and disease. This protocol studies isolated T4 phages within a mouse model, adaptable to other phage-bacterial pairs.

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