T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo

1.4K Views

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08:46 min

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January 26th, 2024

DOI :

10.3791/65906-v

January 26th, 2024

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Nicola Pett¹, Michael Hunter¹, Natalia A. Carranza García¹, Jung Hee Seo¹, Samuel R. Collins¹, Forest Rohwer², Lisa C. Osborne¹, Carolina Tropini¹^,³^,⁴

¹Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, ²Department of Biology, San Diego State University, ³School of Biomedical Engineering, University of British Columbia, ⁴Humans and the Microbiome Program, Canadian Institute for Advanced Research

Summary

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T4 Bacteriophage

E Coli

Host bacteriophage Dynamics

Murine Intestine

Gut Microbiome

Gnotobiotic Mouse

Tripartite Interactions

Phageome Dysregulation

Inflammatory Bowel Disease

Bacterial Evolution

Antimicrobial Resistance

Phage Research Protocols

Microbiota Regulation

Fecal Filtrate Transplants

Chapters in this video

0:00

Introduction

0:54

Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

6:08

In Vivo Monitoring and Quantification of T4 Phage in Mice Using Fecal Spot Plating Assays

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