The Catholic University of Korea, Republic of Korea

5 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Yeri Alice Rim^1,3, Narae Park¹, Yoojun Nam¹, Ji Hyeon Ju^2,3

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, Republic of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, Republic of Korea, ³College of Medicine, The Catholic University of Korea, Republic of Korea

Here we describe a protocol for generating human induced-pluripotent stem cells from patient-derived fibroblast-like synoviocytes, using a lentiviral system without feeder cells.

Developmental Biology

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Yeri Alice Rim¹, Yoojun Nam¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.

Developmental Biology

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Yeonsue Jang¹, Hyerin Jung¹, Ji Hyeon Ju¹

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea

Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

Developmental Biology

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Yoojun Nam¹, Yeri Alice Rim¹, Ji Hyeon Ju²

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

Here, we propose a protocol for chondrogenic differentiation from cord blood mononuclear cell-derived human induced pluripotent stem cells.

Developmental Biology

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim^1,2, Ji Hyeon Ju^1,2,3

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.